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Isoelectric Separation

Application ID: 10626

This example applies the Transport of Diluted Species, Electrostatics, and Laminar Flow interfaces to model isoelectric separation in a free flow electrophoresis device. A stream containing six different ionic species is shown to be divided into pure component streams by means of migrative transport in an electric field.

Free flow electrophoresis can separate macromolecules such as proteins, based on their mobility perpendicular to the flow of the carrier fluid. If, in addition, a pH gradient is applied across the carrier flow, then molecules can be focussed along their isoelectric points. The isoelectric point is the pH at which the molecule has zero net charge. Molecules with a positive net charge will travel in the direction of the electric field, along the pH gradient, until they reach the isoelectric point. At this instance the migrative transport is switched off as the molecules net charge is zero. Similarly, anionic species travel in the direction opposite of the electric field.

This model is included as an example in the following products:

Chemical Reaction Engineering Module

The combination of COMSOL® products required to model your application depends on several factors and may include boundary conditions, material properties, physics interfaces, and part libraries. Particular functionality may be common to several products. To determine the right combination of products for your modeling needs, review the Specification Chart and make use of a free evaluation license. The COMSOL Sales and Support teams are available for answering any questions you may have regarding this.